Immunohistochemistry & Immunofluorescence – Biomarker Analysis on Protein level

Immunohistochemistry (IHC)

Our IHC custom tailored services

In addition to our standard IHC service we do offer custom tailored IHC services.

Our custom services include:

  • Antibody and target selection for your specific study
  • Companion diagnostic validation
  • Validation for translational and clinical research
  • Biomarker screening, using commercial and proprietary antibodies alike

Quality matters

Whether for our standard IHC services or for our custom tailored study – quality matters to us. To ensure the best possible validation of an antibody or a staining we do have the following controls available that can be included in your experiment if desired:

  • Isotype controls
  • Positive control tissues: We do have a large collection of various tissue controls derived from different species with complete organ sets that can be chosen and included as a positive control for your target of choice
  • Negative control tissue
  • Co-staining (double staining or consecutive sections) with an already established marker to evaluate the localisation of a so far not very well characterized target
  • Further validation of the expression of the target on mRNA level -> please click here for more information on our in situ-hybridization or DNA/RNA services

We never stop evolving …

We constantly keep an eye on novel developments in the field. Currently we are implementing a newly developed (Roche, Ventana Medical Systems) approach of multiplexing up to 5 antibodies, using chromogenic detection on our Ventana Discovery Ultra platforms.

Immunofluorescence (IF)

Fluorescence is a real alternative to chromogenic approaches to immunochemistry (IHC). Its High Dynamic Range makes it easier to visualise rare and highly abundant targets in protein localisation studies on the same slide. Moreover, it offers better visualisation of co-localisation of targets with multiple colour identification in multiplexing assays.

Due to the latest developments in fluorophore and microscope technology, the selection of colours used for both single- and multi-colour fluorescence microscopy are greater than ever.

We are highly experienced in direct or indirect detection methods on samples from animal models and tissue. Our laboratory routinely uses fluorescent IHC detection to identify e.g. small amounts of biomarkers or to reveal precise subcellular localisation.